Bioctalysis, Instituto de Catálisis y Petroleoquímica, CSIC, 2 Marie Curie, 28049 Madrid, Spain.
Chemphyschem. 2013 Apr 15;14(6):1237-44. doi: 10.1002/cphc.201201034. Epub 2013 Mar 1.
The biofilm formation of a strain of the extremophile bacterium Acidiphilium sp., capable of donating electrons directly to electrodes, was studied by different surface characterization techniques. We develop a method that allows the simultaneous study of bacterial biofilms by means of fluorescence microscopy and atomic force microscopy (AFM), in which transparent graphitic flakes deposited on a glass substrate are used as a support for the biofilm. The majority of the cells present on the surface were viable, and the growth of the biofilms over time showed a critical increase of the extracellular polymeric substances (EPS) as well as the formation of nanosized particles inside the biofilm. Also, the presence of Fe in Acidiphilium biofilms was determined by X-ray photoelectron spectroscopy (XPS), whereas surface-enhanced infrared absorption spectroscopy indicated the presence of redox-active proteins.
采用不同的表面特性分析技术，研究了可直接向电极传递电子的极端微生物嗜酸硫杆菌（Acidiphilium sp.）的生物膜形成过程。我们开发了一种方法，通过荧光显微镜和原子力显微镜（AFM）同时研究细菌生物膜，其中在玻璃基底上沉积的透明石墨薄片作为生物膜的支撑。表面上存在的大多数细胞是有活力的，随着时间的推移，生物膜的生长显示出细胞外聚合物（EPS）的临界增加，以及生物膜内部纳米颗粒的形成。此外，通过 X 射线光电子能谱（XPS）确定了嗜酸硫杆菌生物膜中的铁的存在，而表面增强红外吸收光谱则表明存在氧化还原活性蛋白。
