Department of Dermatovenereology, West China Hospital of Sichuan University, Chengdu, Sichuan, China.
PLoS One. 2013;8(2):e56830. doi: 10.1371/journal.pone.0056830. Epub 2013 Feb 15.
Our previous work indicated that TWEAK is associated with various types of cutaneous vasculitis (CV). Herein, we investigate the effects of TWEAK on vascular injury and adhesion molecule expression in CV mice. We showed that TWEAK priming in mice induced a local CV. Furthermore, TWEAK priming also increased the extravasation of FITC-BSA, myeloperoxidase activity and the expression of E-selectin and ICAM-1. Conversely, TWEAK blockade ameliorated the LPS-induced vascular damage, leukocyte infiltrates and adhesion molecules expression in LPS-induced CV. In addition, TWEAK treatment of HDMECs up-regulated E-selectin and ICAM-1 expression at both mRNA and protein levels. TWEAK also enhanced the adhesion of PMNs to HDMECs. Finally, western blot data revealed that TWEAK can induce phosphorylation of p38, JNK and ERK in HDMECs. These data suggest that TWEAK acted as an inducer of E-selectin and ICAM-1 expression in CV mice and HDMECs, may contribute to the development of CV.
我们之前的工作表明 TWEAK 与各种类型的皮肤血管炎（CV）有关。在此，我们研究了 TWEAK 对 CV 小鼠血管损伤和粘附分子表达的影响。结果表明，TWEAK 对小鼠的预处理诱导了局部 CV。此外，TWEAK 预处理还增加了 FITC-BSA 的渗出、髓过氧化物酶活性以及 E-选择素和 ICAM-1 的表达。相反，TWEAK 阻断减轻了 LPS 诱导的 CV 中 LPS 诱导的血管损伤、白细胞浸润和粘附分子表达。此外，TWEAK 处理 HDMECs 可上调 E-选择素和 ICAM-1 的 mRNA 和蛋白水平表达。TWEAK 还增强了 PMN 与 HDMECs 的粘附。最后，Western blot 数据显示 TWEAK 可诱导 HDMECs 中 p38、JNK 和 ERK 的磷酸化。这些数据表明，TWEAK 作为 CV 小鼠和 HDMECs 中 E-选择素和 ICAM-1 表达的诱导剂，可能有助于 CV 的发展。
