[转运蛋白基因fscTI和fscTII在杀假丝菌素/FR-008生物合成簇中的功能]
[Function of transporter genes fscTI and fscTII in the biosynthetic cluster of candicidin/FR-008].
作者信息
Lei Xuan, Kong Lingxin, Zhang Chen, You Delin, Deng Zixin
机构信息
State Key Laboratory of Microbial Metabolism and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200030, China.
出版信息
Wei Sheng Wu Xue Bao. 2012 Dec 4;52(12):1458-66.
UNLABELLED
OBJECTIVE To investigate function of transporter genes fscTI and fscTII in the biosynthetic gene cluster of candicidin/FR-008.
METHODS
We constructed a plasmid pJTU4137 for disruption of transporter genes fscTI and fscTII by conjugation and homologous recombinant. The transporter genes were also PCR amplified and cloned into the high-copy plasmid pJTU1278 for overexpression in strain ZYJ-6 derived from Streptomyces sp. FR-008.
RESULTS
The disruption mutant LX10 was unable to produce candicidin and its analogues. Overexpression of FscTI and FscTII in ZYJ-6 caused a 1.5-fold increase in FR-008-III production compared with the control.
CONCLUSION
We confirmed that fscTI and fscTII are function as ATP dependent ATP binding cassetle (ABC) transporters in the biosynthetic gene cluster of FR-008. Furthermore, a positive example was provided for improving antibiotic production in other polyene producing strains based on the results that overexpression of fscTI and fscTI increased candicidin production.
未标记
目的 研究转运蛋白基因fscTI和fscTII在杀假丝菌素/FR-008生物合成基因簇中的功能。
方法
我们构建了质粒pJTU4137，通过接合和同源重组来破坏转运蛋白基因fscTI和fscTII。还通过PCR扩增转运蛋白基因，并将其克隆到高拷贝质粒pJTU1278中，以便在来源于链霉菌属FR-008的菌株ZYJ-6中过表达。
结果
破坏突变体LX10无法产生杀假丝菌素及其类似物。与对照相比，在ZYJ-6中过表达FscTI和FscTII导致FR-008-III产量增加了1.5倍。
结论
我们证实fscTI和fscTII在FR-008生物合成基因簇中作为ATP依赖的ATP结合盒（ABC）转运蛋白发挥作用。此外，基于fscTI和fscTI过表达增加了杀假丝菌素产量的结果，可以为提高其他多烯产生菌株的抗生素产量提供一个积极的例子。
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