Zhukova A A, Prokhorova E E, Tsymbalenko N V, Tokmakova A S, Ataev G L
Parazitologiia. 2012 Sep-Oct;46(5):414-9.
The color of the broodsac sporocyst traditionally serves as the main taxonomic criterion for distinguishing of trematodes of the genus Leucochloridium. Broodsacs of L. paradoxum (Cams, 1835) are green, while broodsacs of L. perturbation (Pojmanska, 1969) are brown. We used molecular genetic analysis of sporocyst rDNA for verifying the accuracy of the mentioned morphological criteria. Trematode infected snails Succinea sp. were collected in Vyritsa and Lyuban (Leningrad Province, Russia). Nucleotide sequences of L. paradoxum (n = 18) and L. perturbatum (n = 10) rDNA including transcribed spacers (ITS1 and ITS2) and 5.8 S rRNA gene were received, rDNA fragments of Leucochloridium sp. sporocysts of the same color were identical. The difference in the ITS1 (2.6%) and ITS2 (6.7%) between sequences of L. paradoxum and L. perturbatum was revealed. Specific nucleotide sequences are deposited at the GeneBank.
传统上，包蚴孢子囊的颜色是区分白吸虫属吸虫的主要分类标准。奇异白吸虫（Cams，1835）的包蚴是绿色的，而扰乱白吸虫（Pojmanska，1969）的包蚴是棕色的。我们使用孢子囊核糖体DNA的分子遗传学分析来验证上述形态学标准的准确性。在维里察和柳班（俄罗斯列宁格勒省）采集了感染吸虫的琥珀螺属蜗牛。获得了奇异白吸虫（n = 18）和扰乱白吸虫（n = 10）核糖体DNA的核苷酸序列，包括转录间隔区（ITS1和ITS2）和5.8 S核糖体RNA基因，相同颜色的白吸虫属孢子囊的核糖体DNA片段是相同的。揭示了奇异白吸虫和扰乱白吸虫序列之间ITS1（2.6%）和ITS2（6.7%）的差异。特定的核苷酸序列已存入基因库。
