School of Pharmacy, Fudan University, Shanghai 201203, China.
J Proteomics. 2013 Apr 26;82:1-13. doi: 10.1016/j.jprot.2013.02.012. Epub 2013 Feb 28.
In proteome research, proteolysis is an important procedure prior to the mass spectrometric identification of proteins. The typical time of conventional in-solution proteolysis is as long as several hours to half a day. To enhance proteolysis efficiency, a variety of microchip bioreactors have been developed for the rapid digestion and identification of proteins in the past decade. This review mainly focuses on the recent advances and the key strategies of microchip bioreactors in protein digestion. The subjects covered include microchip proteolysis systems, the immobilization of proteases in microchannels, the applications of microchip bioreactors in highly efficient proteolysis, and future prospects. It is expected that microchip bioreactors will become powerful tools in protein analysis and will find a wide range of applications in high-throughput protein identification.
在蛋白质组学研究中，蛋白水解是质谱鉴定蛋白质之前的一个重要步骤。传统溶液内蛋白水解的典型时间长达数小时至半天。为了提高蛋白水解效率，在过去十年中，已经开发出了多种微流控生物反应器，用于快速消化和鉴定蛋白质。本综述主要关注微流控生物反应器在蛋白水解方面的最新进展和关键策略。涵盖的主题包括微芯片蛋白水解系统、蛋白酶在微通道中的固定化、微流控生物反应器在高效蛋白水解中的应用以及未来展望。预计微流控生物反应器将成为蛋白质分析的有力工具，并将在高通量蛋白质鉴定中得到广泛应用。
