Department of Life and Nanopharmaceutical Science, Kyung Hee University, Seoul 130-701, Republic of Korea.
Eur J Pharmacol. 2013 Mar 15;704(1-3):70-7. doi: 10.1016/j.ejphar.2013.02.015. Epub 2013 Feb 24.
Salvianolic acid B (SalB) is a polyphenolic compound found in Salvia miltiorrhiza Bunge that has several anti-oxidative and anti-inflammatory effects. In the present study, we investigated whether SalB has neuroprotective effects in an amyloid β (Aβ) peptide-induced Alzheimer's disease mouse model. Mice were injected with Aβ25-35 peptide intracerebroventricularly and were subsequently administered SalB once daily for 7 days. Subchronic SalB administration (10mg/kg) significantly ameliorated the Aβ25-35 peptide-induced memory impairment in the passive avoidance task (P<0.05). SalB treatment also reduced the number of activated microglia and astrocytes that were observed during the inflammatory reaction after the administration of the Aβ25-35 peptide. Moreover, SalB markedly reduced inducible nitric oxide synthase and cyclooxygenase-2 expression levels and thiobarbituric acid reactive substances, which were increased by the administration of the Aβ25-35 peptide. Furthermore, SalB administration significantly rescued the Aβ25-35 peptide-induced decrease of choline acetyltransferase and brain-derived neurotrophic factor protein levels. These results suggest that SalB exerts neuroprotective activity via anti-inflammatory and anti-oxidative effects and that SalB may be a potential candidate for Alzheimer's disease therapy.
丹酚酸 B（SalB）是丹参中的一种多酚化合物，具有多种抗氧化和抗炎作用。在本研究中，我们研究了 SalB 是否对淀粉样β（Aβ）肽诱导的阿尔茨海默病小鼠模型具有神经保护作用。小鼠脑室内注射 Aβ25-35 肽，随后每日给予 SalB 治疗，共 7 天。亚慢性 SalB 给药（10mg/kg）可显著改善 Aβ25-35 肽诱导的被动回避任务中的记忆障碍（P<0.05）。SalB 治疗还减少了 Aβ25-35 肽给药后炎症反应中观察到的活化小胶质细胞和星形胶质细胞的数量。此外，SalB 显著降低了诱导型一氧化氮合酶和环氧化酶-2 的表达水平以及丙二醛反应物质的含量，这些物质在 Aβ25-35 肽给药后增加。此外，SalB 给药可显著挽救 Aβ25-35 肽诱导的胆碱乙酰转移酶和脑源性神经营养因子蛋白水平的降低。这些结果表明，SalB 通过抗炎和抗氧化作用发挥神经保护作用，SalB 可能是治疗阿尔茨海默病的潜在候选药物。
