Schwartz S D, Harrison S A, Engstrom R E, Bawdon R E, Lee D A, Mondino B J
Jules Stein Eye Institute, UCLA Center for Health Sciences 90024-7000.
Am J Ophthalmol. 1990 Jun 15;109(6):701-4. doi: 10.1016/s0002-9394(14)72439-0.
By using a high-pressure liquid chromatography assay, we investigated the ability of collagen shield therapeutic contact lenses to release amphotericin B and deliver it to the anterior segment of rabbit eyes. In vitro studies showed that presoaked collagen shields released most of the amphotericin B within the first hour of elution. We compared the corneal and aqueous humor amphotericin B levels produced by collagen shields soaked in amphotericin B and frequent-drop therapy at four time points over a six-hour period. The collagen shields soaked in amphotericin B produced corneal levels that were higher than those produced by frequent-drop therapy at one hour, equivalent to drop therapy at two and three hours, and lower than drop therapy at six hours. There were no differences in amphotericin B levels in aqueous humor at any time point between rabbits treated with collagen shield delivery and rabbits treated with frequent-drop delivery. The results of this study suggest that amphotericin B delivery to the cornea by collagen shields is comparable to frequent-drop delivery but has the potential benefit of added convenience and compliance.
通过使用高压液相色谱分析方法，我们研究了胶原盾治疗性隐形眼镜释放两性霉素B并将其递送至兔眼前节的能力。体外研究表明，预浸泡的胶原盾在洗脱的第一小时内释放了大部分两性霉素B。我们比较了在六小时内的四个时间点，浸泡在两性霉素B中的胶原盾和频繁滴眼疗法所产生的角膜及房水中两性霉素B的水平。浸泡在两性霉素B中的胶原盾在一小时时产生的角膜药物水平高于频繁滴眼疗法，在两小时和三小时时与滴眼疗法相当，在六小时时低于滴眼疗法。在接受胶原盾给药的兔子和接受频繁滴眼给药的兔子之间，房水中两性霉素B的水平在任何时间点均无差异。本研究结果表明，胶原盾将两性霉素B递送至角膜的效果与频繁滴眼相当，但具有增加便利性和依从性的潜在益处。
