Likhosherstov L M, Piskarev V E, Derevitskaia V A, Kochetkov N K
Bioorg Khim. 1990 Jan;16(1):124-6.
Reaction of beta-oligoglycosylamines obtained from carbohydrate chains of N-glycoproteins (ovalbumin, ovomucoid, riboflavin-binding glycoprotein from hen egg white, and asialofetuin) with bovine serum albumin, lysozyme, and poly(L-Asp) in presence of water-soluble carbodiimide gave rise to a series of glycoconjugates, modelling natural N-glycoproteins. Carbohydrate-peptide bond was shown to be of N-glycosylamide type with participation of Asp and Glu residues. The method allows one to obtain synthetic N-glycoproteins from oligomannoside, complex and hybrid oligosaccharide chains, and may find application both in biochemistry and biotechnology for improvement of physico-chemical properties of unglycosylated proteins.
从N-糖蛋白（卵清蛋白、卵类粘蛋白、鸡卵清中的核黄素结合糖蛋白和去唾液酸胎球蛋白）的碳水化合物链获得的β-寡糖胺与牛血清白蛋白、溶菌酶和聚（L-天冬氨酸）在水溶性碳二亚胺存在下反应，生成了一系列糖缀合物，模拟天然N-糖蛋白。碳水化合物-肽键被证明是N-糖酰胺类型，涉及天冬氨酸和谷氨酸残基。该方法能够从低聚甘露糖苷、复杂和杂合寡糖链获得合成N-糖蛋白，并且可能在生物化学和生物技术领域用于改善未糖基化蛋白质的物理化学性质。
