Key Laboratory of Chemistry of Northwestern Plant Resources, CAS and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China.
J Pharm Biomed Anal. 2013 May 5;78-79:100-4. doi: 10.1016/j.jpba.2013.02.002. Epub 2013 Feb 13.
Transient isotachophoresis (tITP), usually performed in CZE, is a useful on-line sample preconcentration technique for high saline samples. However, only a few papers have applied tITP in MEKC especially SDS system. This study compared tITP in MEKC with that in CZE for 3-nitro-tyrosine (NT) analysis in urine. No sample clean-up was required. Self-stacking occurred simultaneously as chloride in urine acted as a leading ion. HEPES-Tris buffer at pH 8.2 was used as a terminating electrolyte and separation buffer. UV detection wavelength was set at 426 nm. Experiments showed that tITP in MEKC exhibited much higher peak efficiency (up to 1000,000), and improved 4-fold sensitivity, compared with those by tITP in CZE. The limit of detection was 0.07 μM for 3-nitrotyrosine in urine.
瞬时等电聚焦（tITP）通常在 CZE 中进行，是一种用于高盐样品的有用的在线样品浓缩技术。然而，只有少数几篇论文将 tITP 应用于 MEKC 特别是 SDS 体系中。本研究比较了 MEKC 中的 tITP 和 CZE 中的 tITP 在尿液中 3-硝基酪氨酸（NT）分析中的应用。无需样品净化。由于尿液中的氯离子作为先导离子，自堆积同时发生。HEPES-Tris 缓冲液在 pH 8.2 下用作终止电解质和分离缓冲液。UV 检测波长设定为 426nm。实验表明，与 CZE 中的 tITP 相比，MEKC 中的 tITP 表现出更高的峰效率（高达 1000000），并提高了 4 倍的灵敏度。尿液中 3-硝基酪氨酸的检测限为 0.07μM。
