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使用生物标志物、性能协议和数学函数监测慢性身体应激,以识别大鼠的生理适应。

Monitoring chronic physical stress using biomarkers, performance protocols and mathematical functions to identify physiological adaptations in rats.

机构信息

Laboratory of Sports Applied Physiology, Campinas State University (UNICAMP), 13484-350, Limeira, Sao Paulo, Brazil.

出版信息

Lab Anim. 2013 Jan;47(1):36-42. doi: 10.1177/0023677212473715.

DOI:10.1177/0023677212473715
PMID:23467488
Abstract

This study was undertaken to characterize the effects of monotonous training at lactate minimum (LM) intensity on aerobic and anaerobic performances; glycogen concentrations in the soleus muscle, the gastrocnemius muscle and the liver; and creatine kinase (CK), free fatty acids and glucose concentrations in rats. The rats were separated into trained (n = 10), baseline (n = 10) and sedentary (n = 10) groups. The trained group was submitted to the following: 60 min/day, 6 day/week and intensity equivalent to LM during the 12-week training period. The training volume was reduced after four weeks according to a sigmoid function. The total CK (U/L) increased in the trained group after 12 weeks (742.0 ± 158.5) in comparison with the baseline (319.6 ± 40.2) and the sedentary (261.6 ± 42.2) groups. Free fatty acids and glycogen stores (liver, soleus muscle and gastrocnemius muscle) increased after 12 weeks of monotonous training but aerobic and anaerobic performances were unchanged in relation to the sedentary group. The monotonous training at LM increased the level of energy substrates, unchanged aerobic performance, reduced anaerobic capacity and increased the serum CK concentration; however, the rats did not achieve the predicted training volume.

摘要

本研究旨在探讨在乳酸阈(LM)强度下进行单调训练对大鼠有氧运动和无氧运动能力;比目鱼肌、腓肠肌和肝脏中的糖原浓度;以及肌酸激酶(CK)、游离脂肪酸和血糖浓度的影响。将大鼠分为训练组(n=10)、基础组(n=10)和安静组(n=10)。训练组接受以下训练:12 周训练期间,每天 60 分钟,每周 6 天,强度相当于 LM。四周后,根据 sigmoid 函数减少训练量。与基础组(319.6±40.2)和安静组(261.6±42.2)相比,训练 12 周后,训练组的总 CK(U/L)增加(742.0±158.5)。经过 12 周的单调训练后,游离脂肪酸和糖原储存(肝脏、比目鱼肌和腓肠肌)增加,但与安静组相比,有氧运动和无氧运动能力没有变化。在 LM 下进行单调训练会增加能量底物水平,有氧能力不变,无氧能力降低,血清 CK 浓度增加;然而,大鼠并没有达到预期的训练量。

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