SRC 在人结肠癌中酪氨酸 530 位发生去磷酸化。
SRC is dephosphorylated at tyrosine 530 in human colon carcinomas.
机构信息
Department of Biochemistry and Molecular Biology, and Southern Alberta Cancer Research Institute, University of Calgary, Calgary, Alberta, Canada.
出版信息
Chin J Cancer Res. 2011 Sep;23(3):229-31. doi: 10.1007/s11670-011-0229-6.
OBJECTIVE
Src is a protein tyrosine kinase that plays important roles in cancer development, and Src kinase activity has been found to be elevated in several types of cancers. However, the cause of the elevation of Src kinase activity in the majority of human colon carcinomas is still largely unknown. We aim at finding the cause of elevated Src kinase activity in human colon carcinomas.
METHODS
We employed normal colon epithelial FHC cells and examined Src activation in human colon carcinoma specimens from 8 patients. Protein expression levels were determined by Western blotting, and the activity of Src kinase by kinase assay.
RESULTS
Actin levels were different between tumor and normal tissues, demonstrating the complexities and inhomogeneities of the tissue samples. Src kinase activities were increased in the majority of the colon carcinomas as compared with normal colon epithelial cells (range 13-29). Src protein levels were reduced in the colon carcinomas. Src Y530 phosphorylation levels were reduced to a higher extent than protein levels in the carcinomas.
CONCLUSION
The results suggest that Src specific activities were highly increased in human colon carcinomas; phosphorylation at Src Y530 was reduced, contributing to the highly elevated Src specific activity and Src kinase activity.
目的
Src 是一种蛋白酪氨酸激酶，在癌症发展中起着重要作用，并且已经发现几种类型的癌症中 Src 激酶活性升高。然而，大多数人类结肠癌中 Src 激酶活性升高的原因在很大程度上仍不清楚。我们旨在寻找人类结肠癌中 Src 激酶活性升高的原因。
方法
我们使用正常结肠上皮 FHC 细胞，并检查了来自 8 名患者的人结肠癌标本中的 Src 激活情况。通过 Western blot 测定蛋白表达水平，通过激酶测定测定 Src 激酶活性。
结果
肿瘤组织和正常组织之间的肌动蛋白水平不同，表明组织样本的复杂性和不均匀性。与正常结肠上皮细胞相比，大多数结肠癌中 Src 激酶活性升高（范围为 13-29）。Src 蛋白水平在结肠癌中降低。Src Y530 磷酸化水平的降低程度高于癌组织中的蛋白水平。
结论
这些结果表明，人结肠癌中 Src 特定活性高度增加；Src Y530 的磷酸化降低，导致 Src 特定活性和 Src 激酶活性的高度升高。
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