Department of Veterinary Medicine, College of Animal Science, Beijing University of Agriculture, 102206 Beijing, China.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2013;30(4):660-5. doi: 10.1080/19440049.2012.760207. Epub 2013 Mar 8.
Cyromazine and dicyclanil are both used as insect growth regulators. This paper describes an easy and innovative simultaneous extraction method for residues of cyromazine and dicyclanil in food of animal origin, and a confirmation procedure using UHPLC-MS/MS. The sample was extracted, deproteinised by 1% trichloracetic acid in water-acetonitrile, followed by selective defatting using hexane based on the degree of matrix complexity; cleaned-up on an mixed-mode cation exchange (MCX) cartridge; and quantified by using matrix-matched calibration. The mean recoveries were all between 62.0% and 99.2%, and the RSDs were all below 9.94%. The present method was rapid, sensitive and reliable, which was applied to the quantitative analysis of these residues in animal tissues.
环丙氨嗪和双氰胺都被用作昆虫生长调节剂。本文描述了一种简单创新的同时提取动物源食品中环丙氨嗪和双氰胺残留的方法，并采用 UHPLC-MS/MS 进行确证。样品经 1%三氯乙酸在水-乙腈中的提取，根据基质复杂程度用正己烷选择性脱脂；在混合模式阳离子交换（MCX）柱上净化；并用基质匹配校准定量。平均回收率在 62.0%至 99.2%之间，RSD 均低于 9.94%。本方法快速、灵敏、可靠，适用于动物组织中环丙氨嗪和双氰胺残留的定量分析。
