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使用pH梯度平板提高沙门氏菌的耐酸性。

Use of pH gradient plates for increasing the acid tolerance of salmonellae.

作者信息

Huhtanen C N

出版信息

Appl Microbiol. 1975 Mar;29(3):309-12. doi: 10.1128/am.29.3.309-312.1975.

DOI:10.1128/am.29.3.309-312.1975
PMID:234717
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC186971/
Abstract

Several strains of salmonellae survived higher concentrations of lactic acid after streaking on the surface of pH gradient plates. Most strains increased their acid tolerance by about 0.8 to 1.0 pH unit (9-to 10-fold), with Salmonella madelia showing the greatest differential, pH 5.2 in the wild strain and pH 4.2 after conditioning. The increased acid resistance was quickly lost after transferring to normal tryptic soy agar. Tests for survival in a liquid medium at pH values lower than those giving visible growth indicated that these pH values were bactericidal rather than bacteriostatic for both the wild and acid-conditioned strains.

摘要

在pH梯度平板表面划线接种后,有几种沙门氏菌菌株在较高浓度的乳酸中存活了下来。大多数菌株的耐酸性提高了约0.8至1.0个pH单位(9至10倍),其中马德利亚沙门氏菌的差异最大,野生菌株的pH值为5.2,适应后为pH 4.2。转移到普通胰蛋白胨大豆琼脂上后,增加的耐酸性很快就丧失了。在低于可见生长pH值的液体培养基中的存活测试表明,这些pH值对野生菌株和耐酸适应菌株均具有杀菌作用而非抑菌作用。

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本文引用的文献

1
Action of Acetic Acid on Food Spoilage Microörganisms.醋酸对食品腐败微生物的作用
J Bacteriol. 1940 May;39(5):499-515. doi: 10.1128/jb.39.5.499-515.1940.
2
Growth rates of Salmonella colonies.沙门氏菌菌落的生长速率。
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Genetic studies on microbial cross resistance to toxic agents. I. Cross resistance of Escherichia coli to fifteen antibiotics.微生物对有毒物质交叉抗性的遗传学研究。I. 大肠杆菌对十五种抗生素的交叉抗性
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Food-borne salmonellosis.食源性沙门氏菌病
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Microbiology of Lebanon bologna.黎巴嫩博洛尼亚香肠的微生物学
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