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对哥伦比亚马体内十二指肠贾第鞭毛虫的大规模分子研究。

A large scale molecular study of Giardia duodenalis in horses from Colombia.

机构信息

Environmental Microbial and Food Safety Laboratory, ARS, US Department of Agriculture, Beltsville, MD, USA.

出版信息

Vet Parasitol. 2013 Sep 1;196(1-2):31-6. doi: 10.1016/j.vetpar.2013.02.006. Epub 2013 Feb 21.

DOI:10.1016/j.vetpar.2013.02.006
PMID:23474231
Abstract

The prevalence of Giardia duodenalis assemblages in horses is poorly known. The present study examined feces from 195 horses, 1 month-17 years of age, in 4 locations in Colombia. Prevalence of infection was determined by PCR and all positives were sequenced to determine the genotypes. Thirty four (17.4%) horses were found positive. This is the first report of G. duodenalis in horses from Colombia. Prevalence in female and male horses was 18.9% and 15.1%, respectively. Prevalence in horses <1 year of age and horses >1 year of age was 21.1% and 15.1%, respectively. Molecular characterization using the beta giardin (bg), glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi), and small subunit ribosomal RNA (ssurRNA) genes identified G. duodenalis Assemblages A and B, the assemblages regarded as zoonotic.

摘要

贾第虫属在马中的流行情况知之甚少。本研究检查了来自哥伦比亚 4 个地点的 195 匹马,年龄在 1 个月至 17 岁之间。通过 PCR 确定感染的患病率,所有阳性结果均进行测序以确定基因型。发现 34 (17.4%)匹马呈阳性。这是哥伦比亚首次报告马中的贾第虫属。雌性和雄性马的患病率分别为 18.9%和 15.1%。1 岁以下和 1 岁以上的马的患病率分别为 21.1%和 15.1%。使用β-微管蛋白(bg)、谷氨酸脱氢酶(gdh)、磷酸丙糖异构酶(tpi)和小亚基核糖体 RNA(ssurRNA)基因的分子特征鉴定了被认为是人畜共患病的贾第虫属 A 和 B 组。

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