Key Laboratory of Ecology and Environmental Science of Guangdong Higher Education Institutes, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, School of Life Science, South China Normal University, Guangzhou 510631, People's Republic of China.
Comp Biochem Physiol C Toxicol Pharmacol. 2013 May;157(4):366-71. doi: 10.1016/j.cbpc.2013.03.001. Epub 2013 Mar 6.
Apoptotic cell ratio and mRNA expression of caspase-3, cathepsin B (CTSB), heat shock protein 70 (HSP70), manganese superoxide dismutase (MnSOD), catalase (CAT), glutathione peroxidase (GPx) and thioredoxin (TRx) in hemocytes of white shrimp Litopenaeus vannamei exposed to nitrite-N (20 mg/L) was investigated at different stress time (0, 4, 8, 12, 24, 48 and 72 h). The apoptotic cell ratio and mRNA expression level of CTSB were significantly increased in shrimp exposed to nitrite-N for 48 and 72 h. Caspase-3 mRNA expression level significantly increased by 766.50% and 1811.16% for 24 and 48 h exposure, respectively. HSP70 expression level significantly increased at 8 and 72 h exposure. MnSOD mRNA expression in hemocytes up-regulated at 8 and 48 h, while CAT mRNA expression level increased at 24 and 48 h. GPx expression showed a trend that increased first and then decreased. Significant increases of GPx expression were observed at 8 and 12 h exposure. Expression level of TRx reached its highest level after 48 h exposure. These results suggest that nitrite exposure induces expression of apoptosis-related genes in hemocytes, and subsequently caused hemocyte apoptosis. Meanwhile, expression levels of HSP70 and antioxidant enzymes up-regulated to protect the hemocyte against nitrite stress.
研究了亚硝酸盐-N（20mg/L）暴露不同应激时间（0、4、8、12、24、48 和 72h）对凡纳滨对虾（Litopenaeus vannamei）血细胞中细胞凋亡率和半胱天冬酶-3（caspase-3）、组织蛋白酶 B（cathepsin B，CTSB）、热休克蛋白 70（heat shock protein 70，HSP70）、锰超氧化物歧化酶（manganese superoxide dismutase，MnSOD）、过氧化氢酶（catalase，CAT）、谷胱甘肽过氧化物酶（glutathione peroxidase，GPx）和硫氧还蛋白（thioredoxin，TRx）mRNA 表达的影响。结果表明，亚硝酸盐-N 暴露 48 和 72h 后血细胞的细胞凋亡率和 CTSB mRNA 表达水平显著升高；暴露 24 和 48h 后 caspase-3 mRNA 表达水平分别显著增加了 766.50%和 1811.16%；HSP70 表达水平在 8 和 72h 暴露时显著升高；MnSOD mRNA 在血细胞中的表达在 8 和 48h 时上调，CAT mRNA 表达水平在 24 和 48h 时增加；GPx 表达呈先增后降的趋势，在 8 和 12h 暴露时表达显著增加；TRx 表达水平在 48h 暴露后达到最高水平。这些结果表明，亚硝酸盐暴露诱导血细胞中凋亡相关基因的表达，进而导致血细胞凋亡。同时，HSP70 和抗氧化酶的表达水平上调，以保护血细胞免受亚硝酸盐胁迫。
