College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan, 250014, PR China.
J Inorg Biochem. 2013 May;122:49-56. doi: 10.1016/j.jinorgbio.2013.01.010. Epub 2013 Jan 26.
A family of Phterpy complexes, [Mn(Phterpy)2][N(CN)2]2·0.5H2O (1), Fe(Phterpy)22 (2), Ni(Phterpy)22 (3), [Ni(Phterpy)2]Cl2·10H2O (4), Cd(Phterpy)22·2H2O (5) and [Zn(Phterpy)Cl2] (6) (Phterpy=4'-phenyl-2,2':6',2″-terpyridine), have been synthesized and structurally characterized, and their DNA binding and photo-induced DNA cleavage activities have been investigated. These complexes display binding propensity to the CT-DNA giving a relative order: 1>4>3, 5, 2, 6. Under dark or ambient lighting conditions, all complexes show no efficient DNA cleavage activity to pBR322 DNA. While on irradiation with UV-A light of 365nm, complexes of 1, 3 and 4 exhibit significant cleavage activities. In the presence of H2O2 as a revulsant or an activator, the cleavage ability of complex 2 is obviously enhanced. Complexes 5 and 6 do not exhibit any apparent chemical nuclease activities under irradiation conditions or with the addition of H2O2. The DNA photo-induced cleavage activities are consistent with the number of single-electron in the central metal ion of complexes and singlet oxygen and hydroxyl radical are found as the reactive oxygen species.
一个 Phterpy 配合物家族，[Mn(Phterpy)2][N(CN)2]2·0.5H2O（1），Fe(Phterpy)22（2），Ni(Phterpy)22（3），[Ni(Phterpy)2]Cl2·10H2O（4），Cd(Phterpy)22·2H2O（5）和[Zn(Phterpy)Cl2]（6）（Phterpy=4'-苯基-2,2':6',2″-三联吡啶），已经被合成并进行了结构表征，并研究了它们与 DNA 的结合和光诱导 DNA 断裂活性。这些配合物显示出与 CT-DNA 的结合倾向，给出了相对顺序：1>4>3,5,2,6。在黑暗或环境光照条件下，所有配合物对 pBR322 DNA 都没有有效的 DNA 切割活性。然而，在 365nm 的 UV-A 光照射下，1、3 和 4 的配合物表现出显著的切割活性。在 H2O2 作为抑制剂或激活剂存在的情况下，配合物 2 的切割能力明显增强。配合物 5 和 6 在光照条件下或添加 H2O2 时都没有表现出明显的化学核酸酶活性。DNA 光诱导的切割活性与配合物中心金属离子中单电子的数量一致，并发现单线态氧和羟基自由基是反应性氧物种。
