Department 1 Analytical Chemistry; Reference Materials, Federal Institute for Materials Research and Testing (BAM), Berlin, Germany.
Anal Bioanal Chem. 2013 May;405(14):4755-63. doi: 10.1007/s00216-013-6857-6. Epub 2013 Mar 10.
Deoxynivalenol (DON), nivalenol (NIV) and zearalenone (ZEN) are toxic secondary metabolites produced by several species of Fusarium fungi. These mycotoxins are often found together in a large variety of cereal-based foods, which are regulated by maximum content levels of DON and ZEN. To date, suitable certified reference materials (CRM) intended for quality control purposes are lacking for these Fusarium mycotoxins. In order to overcome this lack, the first CRM for the determination of DON, NIV and ZEN in naturally contaminated wheat flour (ERM®-BC600) was developed in the framework of a European Reference Materials (ERM®) project. This article describes and discusses the whole process of ERM®-BC600 development, including material preparation, homogeneity and stability studies, and an interlaboratory comparison study for certification. A total of 21 selected expert laboratories from different European countries with documented expertise in the field of mycotoxin analysis took part in the certification study using various gas and liquid chromatographic methods. The certified values and their corresponding expanded uncertainties (k = 2) were assigned in full compliance with the requirements of ISO Guide 35 and are as follows: 102 ± 11 μg kg(-1) for DON, 1000 ± 130 μg kg(-1) for NIV and 90 ± 8 μg kg(-1) for ZEN.
脱氧雪腐镰刀菌烯醇（DON）、雪腐镰刀菌烯醇（NIV）和玉米赤霉烯酮（ZEN）是几种镰刀菌真菌产生的有毒次生代谢物。这些霉菌毒素通常在各种谷物为基础的食物中一起发现，这些食物受 DON 和 ZEN 最大含量水平的监管。迄今为止，这些镰刀菌霉菌毒素缺乏用于质量控制目的的合适认证参考材料（CRM）。为了克服这一不足，在欧洲参考材料（ERM®）项目框架内，开发了用于测定天然污染小麦粉中 DON、NIV 和 ZEN 的第一个 CRM（ERM®-BC600）。本文描述并讨论了 ERM®-BC600 开发的整个过程，包括材料准备、均匀性和稳定性研究，以及认证的实验室间比较研究。共有 21 家来自不同欧洲国家的选定专家实验室参加了使用各种气相和液相色谱方法进行的认证研究，这些实验室在霉菌毒素分析领域具有文件证明的专业知识。根据 ISO 指南 35 的要求，充分赋值并分配了认证值及其相应的扩展不确定度（k = 2），如下所示：DON 为 102 ± 11 μg kg(-1)，NIV 为 1000 ± 130 μg kg(-1)，ZEN 为 90 ± 8 μg kg(-1)。
