Rapid LC-MS drug metabolite profiling using bioreactor particles.

Enzyme-coated magnetic bioreactor particles enable a fast, convenient approach to metabolic screening. A semi-automated metabolite-profiling technique using these particles in a 96-well plate with liquid chromatography (LC)-mass spectrometry (MS)/MS detection is described. Reactions can be investigated over 1- to 2-min periods, and 96 or more reactions or reaction time points can be processed in parallel. Incorporation of DNA in the particle films facilitates determination of rates of DNA damage and metabolite-DNA adduct structures.