Edwards Mina, Houseman Lyndsey, Phillips Ian R, Shephard Elizabeth A
Institute of Structural and Molecular Biology, University College London, London, UK.
Methods Mol Biol. 2013;987:283-93. doi: 10.1007/978-1-62703-321-3_24.
Primary hepatocyte cultures better reflect the properties of the liver in vivo than do cell lines derived from the liver. Here we describe a method for the isolation and culture of mouse primary hepatocytes. The cells are viable, can be transfected by DNA, and retain key properties of liver cells such as the induction of cytochrome P450 gene expression by drugs such as phenobarbital.
原代肝细胞培养比源自肝脏的细胞系能更好地反映肝脏在体内的特性。在此，我们描述一种分离和培养小鼠原代肝细胞的方法。这些细胞具有活性，可被DNA转染，并保留肝细胞的关键特性，如被苯巴比妥等药物诱导细胞色素P450基因表达。
