Agrawal S, Agarwal S S
Department of Medical Genetics, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow.
Indian J Med Res. 1990 Feb;92:38-42.
Semipurified saline extracts of seeds from Crotolaria juncea, Cassia marginata, Ficus racemosa, Cicer arietinum (L-532), Gossipium indicum (G-27), Melia composita, Acacia lenticularis, Meletia ovalifolia, Acacia catechu and Peltophorum ferrenginium were tested for leukoagglutinating activity against whole leukocytes and mononuclear cells from patients with chronic myeloid leukaemia (34), acute myeloblastic leukaemia (5), acute lymphoblastic leukaemia (7), chronic lymphocytic leukaemia (3), various lymphoproliferative/haematologic disorders (54), and normal healthy subjects (50). In addition, bone marrow cells from three patients undergoing diagnostic bone marrow aspiration and activated lymphocytes from mixed lymphocyte cultures (MLC) were also tested. All the seed extracts agglutinated white blood cells from patients with different types of leukaemia. But none of them reacted with peripheral blood cells of normal individuals, patients with various lymphoproliferative/haematologic disorders or cells from MLC. Leukoagglutination of leukaemic cells with each of the seed extracts was inhibited by simple sugars. Only in one instance, cells from bone marrow of an individual who had undergone diagnostic bone marrow aspiration for a non-malignant condition were agglutinated. It is felt that purification of these seed extracts may yield leukaemia-specific lectins.
对来自菽麻、边缘决明、无花果、鹰嘴豆（L - 532）、印度棉花（G - 27）、复合楝、荚蒾叶相思树、椭圆叶豆梨、儿茶金合欢和铁刀木种子的半纯化盐提取物进行了测试，检测其对慢性粒细胞白血病患者（34例）、急性髓细胞白血病患者（5例）、急性淋巴细胞白血病患者（7例）、慢性淋巴细胞白血病患者（3例）、各种淋巴增殖性/血液系统疾病患者（54例）以及正常健康受试者（50例）的全白细胞和单核细胞的白细胞凝集活性。此外，还对3例接受诊断性骨髓穿刺的患者的骨髓细胞以及混合淋巴细胞培养（MLC）中的活化淋巴细胞进行了测试。所有种子提取物均能凝集不同类型白血病患者的白细胞。但它们均未与正常个体的外周血细胞、各种淋巴增殖性/血液系统疾病患者的细胞或MLC中的细胞发生反应。白血病细胞与每种种子提取物的白细胞凝集反应均被单糖抑制。仅在1例中，1名因非恶性疾病接受诊断性骨髓穿刺的个体的骨髓细胞发生了凝集。人们认为，纯化这些种子提取物可能会产生白血病特异性凝集素。
