Huang Hao, Chen Wei-Wei, Yu Yun, Li Zhan-Yong, Lin Duo, Huang Rong-Zong
College of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China.
Zhongguo Zhong Yao Za Zhi. 2012 Dec;37(23):3569-72.
To test and analyze chemical composition of Paeoniae Radix Alba decoction at molecular level.
Raman spectra of decoctions prepared from seven batches of Paeoniae Radix Alba pieces, five batches of Paeoniae Radix Alba medicinal material and five batches of Paeoniae Radix Rubra pieces were measured respectively, and the characteristic Raman bands were tentatively assigned. Raman spectra of decoctions were compared and analysed between Paeoniae Radix Alba pieces and Paeoniae Radix Alba medicinal material, Paeoniae Radix Alba pieces and Paeoniae Radix Rubra pieces.
Nine Raman bands (637, 783, 847, 981, 1 091, 1 128, 1 336, 1 458 and 1 636 cm(-1)) were observed in the Paeoniae Radix Alba pieces decoction. Compared with the decoction of Paeoniae Radix Alba pieces, the Raman bands of 783, 981, 1 128, 1 336 and 1 458 cm(-1) were also exists in Paeoniae Radix Alba medicinal material decoction, meanwhile, the Raman bands of 633 cm(-1) and 1 633 cm(-1) occurs slight frequency shift, however, the bands of 716, 737, 835, 916, 1 072, 1 271 and 1 600 cm(-1) were disappear. In addition, the Raman spectra are quite different between the decoctions of Paeoniae Radix Alba pieces and Paeoniae Radix Rubra pieces.
The results showed that Raman spectroscopy might provide a novel method for the fast component detection of the Radix Paeoniae Alba decoction or other traditional Chinese medicine.
在分子水平上检测和分析白芍汤的化学成分。
分别测定七批白芍饮片、五批白芍药材和五批赤芍饮片制备的汤剂的拉曼光谱，并对特征拉曼谱带进行初步归属。比较分析白芍饮片与白芍药材、白芍饮片与赤芍饮片汤剂的拉曼光谱。
白芍饮片汤剂中观察到9个拉曼谱带（637、783、847、981、1091、1128、1336、1458和1636cm⁻¹）。与白芍饮片汤剂相比，白芍药材汤剂中也存在783、981、1128、1336和1458cm⁻¹的拉曼谱带，同时，633cm⁻¹和1633cm⁻¹的拉曼谱带发生轻微频移，而716、737、835、916、1072、1271和1600cm⁻¹的谱带消失。此外，白芍饮片与赤芍饮片汤剂的拉曼光谱差异较大。
结果表明，拉曼光谱可能为白芍汤或其他中药的快速成分检测提供一种新方法。
