Wu Hai-Jian, Gong Xiu, Yang Yi-Tian, Wang Yu-Zhuo, Li Xia, Xin Hua, Hu Li-Kuan
Cancer Center, Qilu Hospital, Shandong University, Ji'nan 250012, China.
Zhongguo Zhong Yao Za Zhi. 2012 Dec;37(23):3633-6.
To establish an ideal CCl4 drug-induced liver injury model in vitro.
Traditional method and improved method were adopted for preparing CCl4 injury liquid and drug-induced human liver HepG2 cell injury. Cell morphological change was observed under a bright-field microscope. The level of alanine aminotransferase (ALT) in supernatant was detected by biochemical method. 4-Methyl-tetrazolium (MTT) chromatometry was adopted for determining cell activity.
The improved method showed better CCl4-induced injury effect than the traditional method. With the increase in the concentration of CCl4 injury liquid, the ALT level significantly increased, whereas the cell activity notably decreased. Particularly, 70% CCl4 injury liquid use for 4 hours could achieve the best injury effect.
The improved method could be used to establish an ideal CCl4 drug-induced liver injury model in vitro, which can lay foundation for further in vitro studies.
建立理想的体外四氯化碳药物性肝损伤模型。
采用传统方法和改良方法制备四氯化碳损伤液并诱导人肝HepG2细胞损伤。在明场显微镜下观察细胞形态变化。采用生化方法检测上清液中丙氨酸氨基转移酶（ALT）水平。采用四甲基偶氮唑盐（MTT）比色法测定细胞活性。
改良方法显示出比传统方法更好的四氯化碳诱导损伤效果。随着四氯化碳损伤液浓度的增加，ALT水平显著升高，而细胞活性明显降低。特别地，使用70%四氯化碳损伤液作用4小时可达到最佳损伤效果。
改良方法可用于建立理想的体外四氯化碳药物性肝损伤模型，可为进一步的体外研究奠定基础。
