[超高效液相色谱-四极杆飞行时间质谱法分析川芎嗪吸收进入大鼠脑组织的化学成分]
[Analysis of chemical constituents of chuanxiong rhizoma absorbed into rat brain tissues by UPLC-Q-TOF-MS].
作者信息
Zuo Ai-Hua, Cheng Meng-Chun, Wang Li, Xiao Hong-Bin
机构信息
Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian 116023, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2012 Dec;37(23):3647-50.
OBJECTIVE
To analyze and identify the chemical constituents in rat brain tissues after oral administration of Chuanxiong Rhizoma extracts.
METHOD
The dosed and blank rat brain tissues were analyzed by UPLC-Q-TOF-MS. Different peaks were observed in total ion chromatograms and then identified according to their retention time, accurate mass weight, MS and MS/MS data.
RESULT
After oral administration of Chuanxiong Rhizoma extracts, 3 compounds were absorbed into rat brain tissues through BBB. They were identified as senkyunolide I, senkyunolide A and ligustilide.
CONCLUSION
The study is helpful for interpreting effective substance of Ligusticum chuanxiong.
目的
分析并鉴定口服川芎提取物后大鼠脑组织中的化学成分。
方法
采用超高效液相色谱-四极杆飞行时间质谱联用仪（UPLC-Q-TOF-MS）对给药大鼠和空白大鼠的脑组织进行分析。在总离子色谱图中观察到不同的峰，然后根据它们的保留时间、精确分子量、质谱和串联质谱数据进行鉴定。
结果
口服川芎提取物后，3种化合物通过血脑屏障被吸收进入大鼠脑组织。它们被鉴定为藁本内酯I、藁本内酯A和川芎嗪。
结论
该研究有助于阐释川芎的有效成分。
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