播种廿五载，收获今日果。

A quarter century of reaping what we SOE.

机构信息

Department of Biochemistry, Emory University School of Medicine.

出版信息

Biotechniques. 2013 Mar;54(3):127-8. doi: 10.2144/000114001.

Abstract

Gene Splicing by Overlap Extension (SOE), described in a BioTechniques Research Report 23 years ago, is still widely used today in spite of changes in technology and scientific fashion. The history of SOE offers practical lessons for those who seek the best techniques, and for those who strive to develop them.

摘要

重叠延伸（SOE）基因拼接，在 23 年前的《生物技术研究报告》中有所描述，尽管技术和科学时尚发生了变化，但时至今日仍被广泛应用。SOE 的历史为那些寻求最佳技术的人和那些努力开发这些技术的人提供了实用的经验教训。