Zhang S, Qi Y, Zhang H, He W, Zhou Q, Gui S, Wang Y
Laboratory of Molecular Biology, Anhui Medical University, Hefei 230032, Anhui, PR China.
Biotech Histochem. 2013 Aug;88(6):281-9. doi: 10.3109/10520295.2013.769633. Epub 2013 Mar 11.
The pineal hormone, melatonin (MLT), has been shown to have therapeutic effects in patients with gastric cancer; however, the mechanisms for the anti-cancer effects are unknown. We investigated the effects of melatonin on cell proliferation, apoptosis, colony formation and cell migration in the gastric adenocarcinoma cell line, SGC7901, using MTT assay, Hoechst 33258 staining, flow cytometry, western blot, caspase-3 activity assay, soft agar colony formation assay, and scratch-wound assay. Our results showed that melatonin could inhibit cell proliferation, colony formation and migration efficiency, and it promoted apoptosis of SGC7901 cells. Our findings suggest that the anti-cancer effects of melatonin may be due to both inhibition of tumor cell proliferation and reduction of the metastatic potential of tumor cells.
松果体激素褪黑素（MLT）已被证明对胃癌患者具有治疗作用；然而，其抗癌作用的机制尚不清楚。我们使用MTT法、Hoechst 33258染色、流式细胞术、蛋白质免疫印迹法、caspase-3活性测定、软琼脂集落形成试验和划痕试验，研究了褪黑素对胃腺癌细胞系SGC7901细胞增殖、凋亡、集落形成和细胞迁移的影响。我们的结果表明，褪黑素可以抑制细胞增殖、集落形成和迁移效率，并促进SGC7901细胞凋亡。我们的研究结果表明，褪黑素的抗癌作用可能是由于抑制肿瘤细胞增殖和降低肿瘤细胞的转移潜能。
