Oetomo S B, Lewis J, Ikegami M, Jobe A H
Department of Pediatrics, Harbor-UCLA Medical Center, Torrance 90509.
J Appl Physiol (1985). 1990 Apr;68(4):1590-6. doi: 10.1152/jappl.1990.68.4.1590.
The effect of exogenous surfactant on endogenous surfactant metabolism was evaluated using a single-lobe treatment strategy to compare effects of treated with untreated lung within the same rabbit. Natural rabbit surfactant, Survanta, or 0.45% NaCl was injected into the left main stem bronchus by use of a Swan-Ganz catheter. Radio-labeled palmitic acid was then given by intravascular injection at two times after surfactant treatment, and the ratios of label incorporation and secretion in the left lower lobe to label incorporation and secretion in the right lung were compared. The treatment procedure resulted in a reasonably uniform surfactant distribution and did not disrupt lobar pulmonary blood flow. Natural rabbit surfactant increased incorporation of palmitate into saturated phosphatidylcholine (Sat PC) approximately 2-fold (P less than 0.01), and secretion of labeled Sat PC increased approximately 2.5-fold in the surfactant-treated left lower lobe relative to the right lung (P less than 0.01). Although Survanta did not alter incorporation, it did increase secretion but not to the same extent as rabbit surfactant (P less than 0.01). Alteration of endogenous surfactant Sat PC metabolism in vivo by surfactant treatments was different from that which would have been predicted by previous in vitro studies.
采用单叶治疗策略评估外源性表面活性剂对内源性表面活性剂代谢的影响，以比较同一只兔体内经治疗肺叶与未经治疗肺叶的效果。使用Swan-Ganz导管将天然兔表面活性剂、固尔苏或0.45%氯化钠注入左主支气管。然后在表面活性剂治疗后的两个时间点通过血管内注射给予放射性标记的棕榈酸，并比较左下叶中标记物掺入和分泌与右肺中标记物掺入和分泌的比率。该治疗程序导致表面活性剂分布相当均匀，且未扰乱肺叶的肺血流。天然兔表面活性剂使棕榈酸掺入饱和磷脂酰胆碱（Sat PC）的量增加约2倍（P<0.01），相对于右肺，经表面活性剂处理的左下叶中标记的Sat PC分泌增加约2.5倍（P<0.01）。尽管固尔苏未改变掺入量，但确实增加了分泌，但程度不如兔表面活性剂（P<0.01）。表面活性剂治疗对体内内源性表面活性剂Sat PC代谢的改变与先前体外研究预测的不同。
