McCready R G, Laishley E J, Krouse H R
Can J Microbiol. 1975 Mar;21(3):235-44. doi: 10.1139/m75-034.
During growth on minimal salts--sucrose media supplemented with various concentrations (10-4-10-2 M) of sodium sulfate, Clostridium pasteurianum grew at a normal rate and only evolved sulfide in late stages of growth on 10-2 M SO4-2-. The evolved sulfide was slightly enriched in 34S as compared to the medium sulfur. On the other hand, sulfide was evolved during growth on all concentrations of sulfite tested. Large normal and inverse isotopic effects were observed in the evolved sulfide during SO3-2- reductions. In contrast, the intracellular sulfur showed much smaller fractionations. The complexity of the isotopic patterns suggests that a dissimilatory sulfite reductase system may be induced by high concentrations of sulfite.
在添加了不同浓度（10⁻⁴ - 10⁻² M）硫酸钠的最低盐 - 蔗糖培养基上生长时，巴氏梭菌以正常速率生长，并且仅在10⁻² M SO₄²⁻ 的生长后期释放硫化物。与培养基中的硫相比，释放出的硫化物中³⁴S略有富集。另一方面，在所测试的所有亚硫酸盐浓度下生长时都会释放硫化物。在SO₃²⁻ 还原过程中，释放出的硫化物中观察到了较大的正常和反向同位素效应。相比之下，细胞内的硫显示出小得多的分馏。同位素模式的复杂性表明，高浓度的亚硫酸盐可能会诱导异化亚硫酸盐还原酶系统。
