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用于研究鼓膜生物力学位移的双帧采集全息耳镜

Holographic otoscope using dual-shot-acquisition for the study of eardrum biomechanical displacements.

作者信息

Flores-Moreno Jorge Mauricio, Mendoza Santoyo Fernando, Estrada Rico Julio Cesar

机构信息

Centro de Investigaciones en Optica A. C., León, Guanajuato, Mexico.

出版信息

Appl Opt. 2013 Mar 10;52(8):1731-42. doi: 10.1364/AO.52.001731.

DOI:10.1364/AO.52.001731
PMID:23478779
Abstract

Recently an optoelectronic holography system was deployed in the clinic with the purpose of quantifying the tympanic membrane (TM) displacements of various mammal species, the objective being the understanding of their middle ear biomechanics. The optoelectronic holography system has an in-line configuration where the data gathered is decoded using lensless digital holography with the Fresnel approximation. This paper presents quantitative data obtained from an acoustically excited postmortem chinchilla's TM. To achieve this we used a robust customized windowed unwrapping method to unwrap the noisy optical phase obtained by subtracting phase maps of two recorded holograms and the results were compared with those obtained when using the unwrapping branch-cut algorithm. Additionally, phase maps obtained by the phase-stepping technique were compared applying both unwrapping methods. For in vivo applications particular emphasis is made on post-processing dual-shot-acquisition of holograms as one of various acquisition strategies and algorithms to diminish measurement error due to heartbeat, breathing, and patient's head motion as well as environment induced mechanical disturbances present in a noncontrolled environment, such as in a clinic. By recording only two holograms representing a stationary and deformed state of eardrum, respectively, we can increase the acquisition speed of the camera used to record faster events happening on the TM surface.

摘要

最近,一种光电全息系统被应用于临床,目的是量化各种哺乳动物物种的鼓膜(TM)位移,目标是了解它们的中耳生物力学。该光电全息系统采用同轴配置,所收集的数据使用基于菲涅耳近似的无透镜数字全息术进行解码。本文展示了从经声学激发的死后龙猫鼓膜获得的定量数据。为实现这一目标,我们使用了一种强大的定制加窗展开方法来展开通过减去两个记录全息图的相位图而获得的有噪声光学相位,并将结果与使用展开分支切割算法时获得的结果进行比较。此外,对通过相位步进技术获得的相位图应用两种展开方法进行了比较。对于体内应用,特别强调了全息图的双脉冲采集后处理,这是各种采集策略和算法之一,以减少由于心跳、呼吸、患者头部运动以及在非受控环境(如诊所)中存在的环境引起的机械干扰而导致的测量误差。通过仅记录分别代表鼓膜静止和变形状态的两个全息图,我们可以提高用于记录鼓膜表面更快发生事件的相机的采集速度。

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Holographic otoscope using dual-shot-acquisition for the study of eardrum biomechanical displacements.用于研究鼓膜生物力学位移的双帧采集全息耳镜
Appl Opt. 2013 Mar 10;52(8):1731-42. doi: 10.1364/AO.52.001731.
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