Zhao Longmei, Hantash Basil M
Escape Therapeutics Inc., San Jose, CA, USA.
Methods Mol Biol. 2013;989:11-9. doi: 10.1007/978-1-62703-330-5_2.
Skin regeneration is intricately controlled by epidermal stem cells. In human skin, the long-lived, slow-cycling, and highly proliferative stem cells are located in the basal layer of the interfollicular epidermis (IFE). The ability to isolate and culture human IFE stem cells (IFESCs) offers fascinating therapeutic potential for skin diseases as well as epithelial tissue engineering. Here we describe a straightforward strategy for generation of β1 integrin(+)/CD24(-) IFESCs from scalp with defined, serum-free, feeder-free medium and collagen I-coated culture plates. The use of defined media throughout isolation and cultivation allows for detailed investigation of the molecular events involved in ESC self-renewal and differentiation as well as provides a safe source for clinical use.
皮肤再生由表皮干细胞精确调控。在人类皮肤中，寿命长、循环缓慢且增殖能力强的干细胞位于毛囊间表皮（IFE）的基底层。分离和培养人IFE干细胞（IFESCs）的能力为皮肤疾病以及上皮组织工程提供了极具吸引力的治疗潜力。在此，我们描述了一种直接的策略，即使用特定的无血清、无饲养层培养基和I型胶原包被的培养板，从头皮中生成β1整合素(+)/CD24(-)的IFESCs。在整个分离和培养过程中使用特定培养基，有助于详细研究与胚胎干细胞自我更新和分化相关的分子事件，同时也为临床应用提供了安全的细胞来源。
