Villani Rehan M, Akyuz Mehmet Deniz, Niessen Michaela T, Niessen Carien M
Department of Dermatology, Cologne Excellence Cluster on Cellular Stress Responses in Aging associated Diseases (CECAD), University of Cologne, Cologne, Germany.
Methods Mol Biol. 2013;989:33-43. doi: 10.1007/978-1-62703-330-5_4.
The epidermis is a multilayered epithelium consisting of multiple different progenitor cell populations, all of which are important to epidermal function. In order to study these populations, several techniques have been developed that enable specific purification of the different progenitor cell populations. The best characterized stem cell population in the epidermis, and likely the most pluripotent, are the quiescent stem cells in the hair follicle bulge. In this chapter, we provide a method for isolating bulge stem cells from skin of adult mice using fluorescence-activated cell sorting of immunofluorescently labeled keratinocytes. We use the cell surface markers CD34 and α6-integrin for the enrichment of bulge stem cells. This method also contains notes on how to adjust the cytometer settings for a reproducible analysis.
表皮是一种多层上皮组织，由多个不同的祖细胞群体组成，所有这些群体对表皮功能都很重要。为了研究这些群体，已经开发了几种技术，能够对不同的祖细胞群体进行特异性纯化。表皮中特征最明确且可能最具多能性的干细胞群体是毛囊隆突中的静止干细胞。在本章中，我们提供了一种使用免疫荧光标记角质形成细胞的荧光激活细胞分选从成年小鼠皮肤中分离隆突干细胞的方法。我们使用细胞表面标志物CD34和α6整合素来富集隆突干细胞。该方法还包含有关如何调整细胞仪设置以进行可重复分析的说明。
