凋亡触发效应：紫外线B照射与酿酒酵母
Apoptosis - Triggering Effects: UVB-irradiation and Saccharomyces cerevisiae.
作者信息
Behzadi Payam, Behzadi Elham
机构信息
MSc, Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Shahr-e-Qods Branch, Tehran, Iran.
出版信息
Maedica (Bucur). 2012 Dec;7(4):315-8.
OBJECTIVES
The pathogenic disturbance of Saccharomyces cerevisiae is known as a rare but invasive nosocomial fungal infection. This survey is focused on the evaluation of apoptosis-triggering effects of UVB-irradiation in Saccharomyces cerevisiae.
MATERIALS AND METHODS
The well-growth colonies of Saccharomyces cerevisiae on Sabouraud Dextrose Agar (SDA) were irradiated within an interval of 10 minutes by UVB-light (302 nm). Subsequently, the harvested DNA molecules of control and UV-exposed yeast colonies were run through the 1% agarose gel electrophoresis comprising the luminescent dye of ethidium bromide.
OUTCOMES
No unusual patterns including DNA laddering bands or smears were detected.
CONCLUSIONS
The applied procedure for UV exposure was not effective for inducing apoptosis in Saccharomyces cerevisiae. So, it needs another UV-radiation protocol for inducing apoptosis phenomenon in Saccharomyces cerevisiae.
目的
酿酒酵母的致病性干扰是一种罕见但具有侵袭性的医院获得性真菌感染。本研究旨在评估紫外线B（UVB）照射对酿酒酵母凋亡触发效应的影响。
材料与方法
将在沙氏葡萄糖琼脂（SDA）上生长良好的酿酒酵母菌落每隔10分钟用UVB光（302nm）照射。随后，将对照和紫外线照射后的酵母菌落收获的DNA分子通过含有溴化乙锭发光染料的1%琼脂糖凝胶电泳进行分析。
结果
未检测到包括DNA梯状条带或涂片在内的异常模式。
结论
所应用的紫外线照射程序对诱导酿酒酵母凋亡无效。因此，需要另一种紫外线辐射方案来诱导酿酒酵母的凋亡现象。
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