Huang Li, Zhang Chun-Lin, Huang Ruo-Yang, Chen Han-Bin
Department of Biology, Guiyang Medical University, Guiyang 550004, China.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2012 Oct 30;30(5):353-6.
The salivary gland polytene chromosomes of Simulium qianense was observed with wild larvae collected from Guiyang, Guizhou Province. Using an upgraded method of preparation, the polytene chromosomes of the larval salivary glands of S. qianense were observed and analysed. The results showed three pairs of chromosomes (2n = 6) arranged from the longest to the shortest. Chromosome I was metacentric while chromosomes II and III were submetacentric. The centromeric regions distinctly expanded and were easily recognized. The nucleolar organizer situated near the centromeric band of the short arm of chromosome II. The Balbiani ring and double bubble all located near the tip of the short arm of chromosome II. There were inversions in some individuals, with an inversion frequency of 33% (32/97). The study revealed constant localization and morphology of the principal landmarks (namey, centromere, nucleolar organizer, Balbiani ring, double bulge, band and puff) which can be considered as distinguishable features of S. qianense.
采用从贵州省贵阳市采集的野生幼虫，对黔蚋唾液腺多线染色体进行了观察。运用改进的制片方法，对黔蚋幼虫唾液腺的多线染色体进行了观察与分析。结果显示有三对染色体（2n = 6），按从最长到最短的顺序排列。染色体Ⅰ为中着丝粒染色体，而染色体Ⅱ和Ⅲ为亚中着丝粒染色体。着丝粒区域明显扩展，易于识别。核仁组织区位于染色体Ⅱ短臂着丝粒带附近。巴尔比亚尼环和双泡均位于染色体Ⅱ短臂末端附近。部分个体存在倒位，倒位频率为33%（32/97）。该研究揭示了主要特征（即着丝粒、核仁组织区、巴尔比亚尼环、双泡、带和胀泡）的恒定定位和形态，这些可被视为黔蚋的显著特征。
