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[日本血吸虫琼脂-石蜡双重包埋纵切片的制备]

[Preparation of agar-paraffin double-embedded longitudinal sections of Schistosoma japonicum].

作者信息

Wang Ke-Geng, Zeng Qing-Ren, Zhang Yu-Kuai, Zhou Jun, Cai Li-Ting, Liang Yu, Liu Yan

机构信息

Department of Parasitology, School of Medicine, University of South China, Hengyang 421001, China.

出版信息

Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2012 Oct 30;30(5):415-7.

Abstract

Schistosoma japonicum adults are pre-embedded in a double-layer agar and made the block, then dehydrated with alcohol, isobutyl alcohol and n-butyl alcohol. Various staining procedures can be conducted after conventional sectioning and dewaxing. Complete longitudinal serial sections of the pre-embedded worms can be obtained, and the desired sections can be easily located accurately.

摘要

日本血吸虫成虫预先包埋于双层琼脂中制成组织块,然后依次用酒精、异丁醇和正丁醇脱水。常规切片和脱蜡后可进行各种染色程序。可以获得预先包埋虫体的完整纵向连续切片,并且可以轻松准确地定位所需切片。

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