Fang Dianji, Guo Yanwei, Li Song, Ning Zhaorong
Dept. of Oral and Maxillofacial Surgery, Hospital of Stomatology, Jiamusi University, Jiamusi 154000, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2013 Feb;31(1):65-9.
To investigate the effect of Eucommiol on osteogenic differentiation of adipose-derived stem cells (ADSCs), and the feasibility of applying in mandibular defects repair using ADSCs combined with the extract of Eucommiol scaffold material.
Forty-eight New Zealand rabbits were randomly divided into four groups and bilateral mandibular defect was prepared. Group A: Implanted the ADSCs combined with the extract of Eucommiol scaffold, group B: Implanted the ADSCs combined with hydroxyapatite materials, group C: Implanted hydroxyapatite materials, group D: The control group. All the experimental animals were sacrificed after 2, 4, 8, 12 weeks. Tissue samples were observed by gross observation, radiographic analysis, hematoxylin-eosin (HE) staining, scanning electron microscope (SEM). The value of imaging analysis and osteogenesis were evaluated. The results were analyzed by SPSS 17.0 statistical software.
In vivo imaging and histological staining showed that the healing of bone defect and bone quality in group A was significantly better than those in the other groups. SEM showed well biocompatibility between composite material and tissue without inflammation reaction. By measuring and analyzing the dental CT data and new bone area, the bone in group A was obviously better than those in other groups (P < 0.05).
The extract of Eucommiol can promote ADSCs into bone cells. The role of ADSCs combined with extract of Eucommiol scaffold materials has significant bone induction. It is expected that this material could become a new composite material and be used to fix the jaw bone defects.
研究杜仲醇对脂肪来源干细胞（ADSCs）成骨分化的影响，以及ADSCs联合杜仲醇提取物支架材料应用于下颌骨缺损修复的可行性。
将48只新西兰兔随机分为4组，制备双侧下颌骨缺损。A组：植入ADSCs联合杜仲醇提取物支架；B组：植入ADSCs联合羟基磷灰石材料；C组：植入羟基磷灰石材料；D组：对照组。所有实验动物在2、4、8、12周后处死。对组织样本进行大体观察、影像学分析、苏木精-伊红（HE）染色、扫描电子显微镜（SEM）观察。评估影像学分析和成骨价值。结果采用SPSS 17.0统计软件进行分析。
体内影像学和组织学染色显示，A组骨缺损愈合情况和骨质量明显优于其他组。SEM显示复合材料与组织具有良好的生物相容性，无炎症反应。通过测量和分析牙科CT数据及新骨面积，A组骨情况明显优于其他组（P<0.05）。
杜仲醇提取物可促进ADSCs向骨细胞分化。ADSCs联合杜仲醇提取物支架材料具有显著的骨诱导作用。有望该材料成为一种新型复合材料用于修复颌骨缺损。
