Duan Xian-Liang, Wang Qin-Ling, Wang Jin-Gang, Wang Chang-Yu, Fan Hua
Department of Hematology, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2013 Feb;21(1):7-11. doi: 10.7534/j.issn.1009-2137.2013.01.002.
This study was purposed to investigate the relationship between expression of the FANCG gene and adult sporadic acute myeloid leukemia (AML), real-time PCR with SYBR Green I technique was used for detecting FANCG gene expression level in bone marrow mononuclear cells of 54 newly diagnosed AML patients, 46 AML patients in complete remission (CR) and 36 control samples. β-actin gene was used as internal reference. Relative changes of FANCG gene expression level were detected by 2(-ΔΔCT) method in newly diagnosed AML patients and control samples, in newly diagnosed AML and patient in CR, as well as in AML patients in CR and control samples. The results showed that the relative expression level of FANCG mRNA was 0.56 ± 0.27 in newly diagnosed group, 0.75 ± 0.54 in AML CR group, and 0.85 ± 0.45 in control group. The expression level of FANCG mRNA in newly diagnosed group was significantly lower than that in control and AML CR groups (P < 0.05). There was no statistically significant deference in comparison of AML CR group with the control group (P > 0.05). It is concluded that the expression of FANCG gene decrease in the newly diagnosed AML patients. There is no significant difference between AML CR group and control group, which indicated that FANCG gene may be related with the onset and the prognosis of AML, and may provide a clinical value for evaluating effect of chemotherapy.
本研究旨在探讨FANCG基因表达与成人散发性急性髓系白血病（AML）的关系，采用SYBR Green I实时荧光定量PCR技术检测54例初诊AML患者、46例完全缓解（CR）的AML患者及36例对照样本骨髓单个核细胞中FANCG基因的表达水平。以β-肌动蛋白基因作为内参。采用2(-ΔΔCT)法检测初诊AML患者与对照样本、初诊AML患者与CR患者以及CR患者与对照样本中FANCG基因表达水平的相对变化。结果显示，初诊组FANCG mRNA相对表达水平为0.56±0.27，AML CR组为0.75±0.54，对照组为0.85±0.45。初诊组FANCG mRNA表达水平显著低于对照组和AML CR组（P<0.05）。AML CR组与对照组比较差异无统计学意义（P>0.05）。结论：初诊AML患者FANCG基因表达降低。AML CR组与对照组无显著差异，提示FANCG基因可能与AML的发病及预后相关，可为评估化疗效果提供临床价值。
