Cell Dynamics Research Group Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, 1-8-31 Midorigaoka, Ikeda, Osaka, 563-8577, Japan +81 72 751 7997 ; +81 72 751 9628 ;
Expert Opin Drug Discov. 2009 Apr;4(4):373-89. doi: 10.1517/17460440902804372.
Bioluminescence technology is based on the luciferin-luciferase reaction and is generally well known as a reporter gene assay system that uses firefly luciferase. It has revolutionized the field of transcriptional analysis owing to its usability and quantitative capability. Several methods for transcription analysis have emerged in the past two decades. Recently, novel bioluminescence techniques that differ from typical approaches were developed for the detection of transcriptional regulation or direct protein-protein interactions.
As each method has its own characteristics, this review summarizes the latest bioluminescence methods that are applicable to the field of drug discovery research.
Considering the diversity of related techniques, this review covers several aspects that have been divided into the following classes: variation of reporter gene assays, secretion properties, protein-protein interaction assays in living cells and bioluminescence imaging of living cells.
RESULTS/CONCLUSIONS: The practical application of several luciferins and/or luciferases and the generation of novel applications by incorporating fluorescent molecules into bioluminescence techniques will become increasingly important because bioluminescence technology has a significant potential depending on how we use it.
生物发光技术基于荧光素-荧光酶反应，通常被称为使用萤火虫荧光素酶的报告基因检测系统，由于其可用性和定量能力，它彻底改变了转录分析领域。在过去的二十年中，已经出现了几种转录分析方法。最近，为了检测转录调控或直接蛋白质-蛋白质相互作用，开发了不同于典型方法的新型生物发光技术。
由于每种方法都有其特点，因此本综述总结了适用于药物发现研究领域的最新生物发光方法。
考虑到相关技术的多样性，本综述涵盖了几个方面，分为以下几类：报告基因检测的变化、分泌特性、活细胞中的蛋白质-蛋白质相互作用检测以及活细胞的生物发光成像。
结果/结论：由于生物发光技术具有很大的潜力，因此几种荧光素和/或荧光酶的实际应用以及通过将荧光分子纳入生物发光技术来产生新的应用将变得越来越重要。
