Wada C, Ohtani H
Department of Clinical Pathology, Kitasato University School of Medicine, Sagamihara.
Rinsho Byori. 1990 Mar;38(3):288-93.
A cDNA complementary to the parathyroid hormone related protein (PTHrP), the humoral hypercalcemia factor in malignancy, was recently isolated and sequenced. PTHrP expression in human carcinomas was examined (determined) by Northern blot hybridization, Southern blot hybridization and radioimmunoassay (RIA). Expression of PTHrP mRNA was detected in three out of four lung squamous cell carcinomas, two out of ten breast carcinomas and the one adenosquamous carcinoma of the maxilla. No clinical hypercalcemia was found in these PTHrP mRNA positive carcinomas. No expression of PTHrP was detected in normal human or rat tissues. In Southern blot hybridization, no amplification of PTHrP gene was found in PTHrP positive cases. An insertion in one allele of promotor region of the gene was identified in one PTHrP positive lung squamous cell carcinoma. The serum level of PTHrP was examined using human PTHrP (1-34) RIA in lung carcinomas. We found no correlation between the level of PTHrP and clinical hypercalcemia or the histopathological diagnosis. We discuss some problems of the PTHrP assay as a novel tumor marker for malignancies. A new RIA assay study using recombinant human PTHrP expressed in Escherichia coli is also reported.
与甲状旁腺激素相关蛋白（PTHrP）（恶性肿瘤中的体液性高钙血症因子）互补的cDNA最近被分离并测序。通过Northern印迹杂交、Southern印迹杂交和放射免疫分析（RIA）检测（确定）了人癌组织中PTHrP的表达。在四例肺鳞状细胞癌中有三例、十例乳腺癌中有两例以及上颌腺鳞癌中检测到了PTHrP mRNA的表达。在这些PTHrP mRNA阳性癌中未发现临床高钙血症。在正常人体或大鼠组织中未检测到PTHrP的表达。在Southern印迹杂交中，在PTHrP阳性病例中未发现PTHrP基因的扩增。在一例PTHrP阳性肺鳞状细胞癌中，在该基因启动子区域的一个等位基因中发现了一个插入。使用人PTHrP（1-34）RIA检测肺癌患者血清中PTHrP的水平。我们发现PTHrP水平与临床高钙血症或组织病理学诊断之间没有相关性。我们讨论了PTHrP检测作为一种新型恶性肿瘤标志物的一些问题。还报道了一项使用在大肠杆菌中表达的重组人PTHrP进行的新的RIA检测研究。
