对单核吞噬细胞系统不同区室中卡介苗肉芽肿内巨噬细胞的研究。

Study of macrophages in BCG granulomas in different compartments of the mononuclear phagocyte system.

作者信息

Arkhipov S A, Shkurupy V A, Solomatina M V, Akhramenko E S, Iljine D A

机构信息

Research Center of Clinical and Experimental Medicine, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia.

出版信息

Bull Exp Biol Med. 2013 Feb;154(4):467-70. doi: 10.1007/s10517-013-1979-6.

DOI:10.1007/s10517-013-1979-6

PMID:23486583

Abstract

We studied in vitro morphological and functional properties of macrophages associated with their M1 and M2 polarization in different mononuclear phagocyte compartments during BCG-induced granuloma formation, namely expression patterns of cytokines IL-1α, GM-CSF, TNF-α, and clusters of differentiation CD36 and CD16/32. We showed the mosaic pattern of macrophage polarization in BCG granulomatosis manifested by simultaneous formation of different macrophage subpopulations with M1 and M2 phenotypes in the population of mononuclear phagocytes of BCG granulomas and various compartments of the mononuclear phagocyte system. These data clarify the role of the functional polarization of macrophages in the pathogenesis of tuberculosis infection.

摘要

我们研究了卡介苗诱导肉芽肿形成过程中，不同单核吞噬细胞区室中与巨噬细胞M1和M2极化相关的体外形态学和功能特性，即细胞因子IL-1α、GM-CSF、TNF-α以及分化簇CD36和CD16/32的表达模式。我们展示了卡介苗肉芽肿病中巨噬细胞极化的镶嵌模式，表现为在卡介苗肉芽肿的单核吞噬细胞群体以及单核吞噬细胞系统的各个区室中，同时形成具有M1和M2表型的不同巨噬细胞亚群。这些数据阐明了巨噬细胞功能极化在结核感染发病机制中的作用。