Xie Xin-Yuan, Sun Pei-De, Lou Ju-Qing, Guo Mao-Xin, Ma Wang-Gang
School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China.
Huan Jing Ke Xue. 2013 Jan;34(1):209-16.
An Acidithiobacillus ferrooxidans strain WZ-1 was isolated from the tannery sludge in Wenzhou, Zhejiang Province in China. The cell of WZ-1 strain is Gram negative and rod-shaped, its 16S rDNA sequence is closely related to that of Acidithiobacillus ferrooxidans ATCC23270 with 99% similarity. These results reveal that WZ-1 is a strain of Acidithiobacillus ferrooxidans. The effects of Ni2+, Cr3+, Cu2+, Zn2+ and 5 kinds of simulated leaching solutions of electroplating sludge on the bioactivity of Fe2+ oxidation and apparent respiratory rate of WZ-1 were investigated. The results showed that Ni2+ and Cr3+ did not have any influence on the bioactivity of WZ-1 at concentrations of 5.0 g x L(-1) and 0.1 g x L(-1), respectively. WZ-1 showed tolerance to high levels of Ni2+, Zn2+ (about 30.0 g x L(-1)), but it had lower tolerance to Cr3+ and Cu2+ (0.1 g x L(-1) Cr3+ and 2.5 g x L(-1) Cu2+). Different kinds of simulated leaching solution of electroplating sludge had significant differences in terms of their effects on the bioactivity of WZ-1 with a sequence of Cu/Ni/Cr/Zn > Cu/Ni/Zn > Cu/Cr/Zn > Cu/Ni/Cr > Ni/Cr/Zn.
从中国浙江省温州市的制革厂污泥中分离出一株氧化亚铁硫杆菌WZ-1。WZ-1菌株的细胞为革兰氏阴性，呈杆状，其16S rDNA序列与氧化亚铁硫杆菌ATCC23270的序列密切相关，相似度为99%。这些结果表明WZ-1是氧化亚铁硫杆菌的一个菌株。研究了Ni2+、Cr3+、Cu2+、Zn2+以及5种电镀污泥模拟浸出液对WZ-1亚铁氧化生物活性和表观呼吸速率的影响。结果表明，Ni2+浓度为5.0 g x L(-1)、Cr3+浓度为0.1 g x L(-1)时，对WZ-1的生物活性均无影响。WZ-1对高浓度的Ni2+、Zn2+（约30.0 g x L(-1)）具有耐受性，但对Cr3+和Cu2+的耐受性较低（0.1 g x L(-1) Cr3+和2.5 g x L(-1) Cu2+）。不同种类的电镀污泥模拟浸出液对WZ-1生物活性的影响存在显著差异，顺序为Cu/Ni/Cr/Zn > Cu/Ni/Zn > Cu/Cr/Zn > Cu/Ni/Cr > Ni/Cr/Zn。
