Siddiqui A A, Zhou Y, Podesta R B, Clarke M W
Department of Zoology, University of Western Ontario, London, Canada.
Mol Biochem Parasitol. 1990 Apr;40(1):95-103. doi: 10.1016/0166-6851(90)90083-x.
A procedure is described whereby a highly enriched plasma membrane fraction was isolated from Trypanosoma brucei by the technique of preparative free-flow electrophoresis. The purity of the plasma membrane fraction was monitored by electron microscopy and by marker enzymology, and is compared to those obtained by previous methods. Proteins associated with plasma membrane fraction were analyzed by SDS-PAGE and phase separated in Triton X-114.
本文描述了一种通过制备型自由流动电泳技术从布氏锥虫中分离出高度富集的质膜组分的方法。通过电子显微镜和标记酶学对质膜组分的纯度进行监测，并与以前方法获得的纯度进行比较。通过SDS-PAGE分析与质膜组分相关的蛋白质，并在Triton X-114中进行相分离。
