Graduate School of Environmental Studies, Tohoku University, Sendai 980-8579, Japan.
Anal Chem. 2013 Apr 16;85(8):3832-5. doi: 10.1021/ac303569t. Epub 2013 Apr 3.
In this study, we fabricated a probe consisting of a carbon nanoelectrode and an Ag/AgCl reference electrode for detecting the activity of cells in single droplets. HeLa cells were confined into a single droplet, and the alkaline phosphatase (ALP) activity of the cells was electrochemically measured using the probe inserted into the droplet. The ALP of the confined cells catalyzed the hydrolysis of p-aminophenyl phosphate (PAPP) to yield p-aminophenol (PAP) that gave electrochemical responses. Since the tip of the carbon-Ag/AgCl probe is very small, it is useful for electrochemical analysis of cells using droplets.
在这项研究中，我们制备了一种由碳纳米电极和 Ag/AgCl 参比电极组成的探针，用于检测单个液滴中细胞的活性。将 HeLa 细胞限制在单个液滴中，并用插入液滴的探针电化学测量细胞的碱性磷酸酶 (ALP) 活性。限制在细胞内的 ALP 催化对氨基苯磷酸 (PAPP) 的水解，生成具有电化学响应的对氨基酚 (PAP)。由于碳-Ag/AgCl 探针的尖端非常小，因此非常适合使用液滴进行细胞电化学分析。
