Instituto de Química de Araraquara, UNESP , Araraquara, São Paulo , Brazil.
J Microencapsul. 2013;30(7):624-31. doi: 10.3109/02652048.2013.770097. Epub 2013 Mar 14.
Microbial enzymes have been used for various biotechnological applications; however, enzyme stabilization remains a challenge for industries and needs to be considered. This study describes the effects of spray-drying conditions on the activity and stability of β-fructofuranosidase from Fusarium graminearum. The extracellular enzyme β-fructofuranosidase was spray dried in the presence of stabilizers, including starch (Capsul) (SC), microcrystalline cellulose (MC), trehalose (TR), lactose (LC) and β-cyclodextrin (CD). In the presence of TR (2% w/v), the enzymatic activity was fully retained. After 1 year of storage, 74% of the enzymatic activity was maintained with the CD stabilizer (10% w/v). The residual activity was maintained as high as 80% for 1 h at 70°C when MC, SC and CD (5% w/v) stabilizers were used. Spray drying with carbohydrates was effective in stabilizing the F. graminearum β-fructofuranosidase, improved enzymatic properties compared to the soluble enzyme and demonstrated a potential use in future biotechnology applications.
微生物酶已被用于各种生物技术应用；然而，酶的稳定性仍然是工业界面临的挑战，需要加以考虑。本研究描述了喷雾干燥条件对禾谷镰刀菌β-呋喃果糖苷酶活性和稳定性的影响。在稳定剂（包括淀粉（Capsul）（SC）、微晶纤维素（MC）、海藻糖（TR）、乳糖（LC）和β-环糊精（CD））存在下，对细胞外酶β-呋喃果糖苷酶进行喷雾干燥。在 TR（2%w/v）存在下，酶活性完全保留。在储存 1 年后，用 CD 稳定剂（10%w/v）保持 74%的酶活。当使用 MC、SC 和 CD（5%w/v）稳定剂时，在 70°C 下 1 小时内，残余活性保持在 80%以上。碳水化合物的喷雾干燥有效地稳定了禾谷镰刀菌β-呋喃果糖苷酶，与可溶性酶相比提高了酶的性质，并显示出在未来生物技术应用中的潜在用途。
