使用寡核苷酸探针进行HLA DQ分型。
Typing for HLA DQ using oligonucleotidic probes.
作者信息
Khalil I, d'Auriol L, Morin L, Gobet M, Lepage V, Galibert F, Degos L, Hors J
机构信息
INSERM U 93, Hôpital St Louis, Paris, France.
出版信息
Nouv Rev Fr Hematol (1978). 1990;32(1):109-12.
PMID:2349076
Abstract
We have typed 25 homozygous B lymphoblastoid cell lines, defined as reference panel during the Xth International Histocompatibility Workshop, using PCR (Polymerase Chain Reaction) and oligonucleotidic probes recognizing sequences of DQA and DQB genes. The polymorphism of 8 HLA-DQA1, and 12 DQB1 alleles is reported and the advantages of this technique are compared to that of other typing methods, currently used.
摘要
我们使用聚合酶链反应（PCR）和识别DQA和DQB基因序列的寡核苷酸探针，对25个纯合B淋巴母细胞系进行了分型，这些细胞系在第十届国际组织相容性研讨会上被定义为参考样本。报告了8个HLA - DQA1和12个DQB1等位基因的多态性，并将该技术的优势与目前使用的其他分型方法的优势进行了比较。
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