Dieye A, Diaw M L, Ndiaye M, Thiam A, Tall A, Spiegel A, Ndiaye R
Laboratoire d'Immunogénétique moléculaire, Institut Pasteur de Dakar.
Dakar Med. 1999;44(1):58-62.
In this study, we compared the serological standard typing method and the DNA genotyping PCR-SSP for the characterization of HLA-DQ alleles in a senegalese population. For this purpose, 120 individuals leaving in Dielmo were typed using the microlymphocytotoxicity assay and the PCR-SSP DQ low Resolution method. A discordance of 42.5% (51/120) was found between these two methods. Thirty % (36/120) of serological typed persons failed to be typed by PCR-SSP method whereas 1% (1/120) assigned by PCR-SSP failed to be characterized by serology. Advantages and limits of these two typing methods and also the genetic background of our study population were valid arguments to comment our findings. The PCR-SSP, as suggested by several authors, is reliable, accurate and fast for HLA class II alleles characterization. Nevertheless, it needs, to become an alternative HLA typing method, available primers adapted to genetic background of study population.
在本研究中，我们比较了血清学标准分型方法和DNA基因分型PCR-SSP，以鉴定塞内加尔人群中的HLA-DQ等位基因。为此，使用微量淋巴细胞毒性试验和PCR-SSP DQ低分辨率方法对居住在迪耶尔莫的120名个体进行分型。这两种方法之间的不一致率为42.5%（51/120）。30%（36/120）血清学分型的人未能通过PCR-SSP方法分型，而PCR-SSP分型的1%（1/120）未能通过血清学鉴定。这两种分型方法的优缺点以及我们研究人群的遗传背景是解释我们研究结果的有效论据。正如几位作者所建议的，PCR-SSP对于HLA II类等位基因的鉴定是可靠、准确且快速的。然而，要成为一种替代的HLA分型方法，它需要有适合研究人群遗传背景的可用引物。
