Hagen G, Wolf M, Katyal S L, Singh G, Beato M, Suske G
Institut für Molekularbiologie und Tumorforschung, Marburg, FRG.
Nucleic Acids Res. 1990 May 25;18(10):2939-46. doi: 10.1093/nar/18.10.2939.
The amino acid sequence of rat Clara Cell 10 kDa secretory protein (CC10) shows 55% identity to rabbit uteroglobin. In order to define the relationship between rat CC10 and rabbit uteroglobin in detail, the tissue-specific expression and hormonal regulation of rat CC10 mRNA was analyzed. We report that like rabbit uteroglobin, rat CC10 mRNA is expressed in lung and esophagus, as well as in uteri of estrogen- and progesterone-treated females. Expression of CC10 mRNA in lung is regulated by glucocorticoids. The similarity in expression pattern of rat CC10 mRNA and rabbit uteroglobin mRNA is reflected by a striking similarity in the 5'-flanking regions of the two genes. Despite this overall similarity, two regions of 0.3 kb and 2.1 kb are absent in the rat CC10 upstream gene region. The larger region includes a cluster of hormone receptor binding sites, believed to be responsible for differential regulation of rabbit uteroglobin by glucocorticoids and progesterone. Thus, while the sequence identities in the coding and 5'-flanking regions point towards a common ancestor for the uteroglobin and CC10 gene, later events (deletions/insertions) might have caused species-specific differences in their regulation.
大鼠克拉拉细胞10 kDa分泌蛋白（CC10）的氨基酸序列与兔子宫珠蛋白有55%的同源性。为了详细确定大鼠CC10与兔子宫珠蛋白之间的关系，对大鼠CC10 mRNA的组织特异性表达和激素调节进行了分析。我们报告，与兔子宫珠蛋白一样，大鼠CC10 mRNA在肺、食管以及经雌激素和孕酮处理的雌性大鼠的子宫中表达。肺中CC10 mRNA的表达受糖皮质激素调节。大鼠CC10 mRNA和兔子宫珠蛋白mRNA表达模式的相似性反映在两个基因5'侧翼区域的显著相似性上。尽管总体相似，但大鼠CC10上游基因区域缺少0.3 kb和2.1 kb的两个区域。较大的区域包括一组激素受体结合位点，据信这些位点负责糖皮质激素和孕酮对兔子宫珠蛋白的差异调节。因此，虽然编码区和5'侧翼区域的序列同源性表明子宫珠蛋白和CC10基因有共同的祖先，但后来的事件（缺失/插入）可能导致了它们在调节上的物种特异性差异。
