伊朗精索静脉曲张相关不育症患者中 POLG 基因多态性。
The POLG Gene Polymorphism in Iranian Varicocele-Associated Infertility Patients.
机构信息
Department of Biology, Science School, Yazd University, Yazd, Iran.
出版信息
Iran J Basic Med Sci. 2012 Mar;15(2):739-44.
OBJECTIVES
Varicocele is associated with impaired testicular function and male infertility, but the molecular mechanisms by which fertility is affected have not been satisfactorily explained. The aim of our study was to investigate whether or not the polymerase gamma (POLG) polymorphism is associated with Iranian varicocele patients.
MATERIALS AND METHODS
We determined the POLG CAG repeat length in DNA samples extracted from 40 varicocele patients and 30 control subjects by PCR-denature polyacrylamide gel electrophoresis and sequencing.
RESULTS
The distribution of the CAG repeat length in varicocele patients showed no notable difference from that in control subjects, but we found a significant statistical inverse correlation between 10/10 and 10/#10 genotypes and varicocele grade.
CONCLUSION
These findings indicate that POLG CAG repeats may affects the varicocele grade, but the mechanisms remain to be elucidated.
目的
精索静脉曲张与睾丸功能障碍和男性不育有关，但导致生育能力受损的确切机制尚不能完全解释。本研究旨在探讨聚合酶γ（POLG）多态性是否与伊朗精索静脉曲张患者有关。
材料和方法
我们通过 PCR-变性聚丙烯酰胺凝胶电泳和测序法，测定了 40 名精索静脉曲张患者和 30 名对照者 DNA 样本中的 POLG CAG 重复长度。
结果
精索静脉曲张患者的 CAG 重复长度分布与对照组无明显差异，但我们发现 10/10 和 10/#10 基因型与精索静脉曲张分级之间存在显著的统计学负相关。
结论
这些发现表明，POLG CAG 重复可能影响精索静脉曲张分级，但机制尚待阐明。
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