Department of Respiratory, The First Hospital, Lanzhou University, Lanzhou 730000, China.
Department of Immunology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Iran J Basic Med Sci. 2014 Feb;17(2):128-33.
OBJECTIVE(S): Accumulating evidence has demonstrated that miRNAs contribute to various genetic and epigenetic modifications in the pathogenesis of gastric cancer (GC). Recent studies focused on the four single nucleotide polymorphisms (SNPs) of pre-miRNAs including rs11614913, rs3746444, rs2910164, and rs2292832. It was suggested that these four SNPs were significantly associated with the risk of GC and were described as candidate susceptibility factors. However, the previous results show conflicting findings. The aim of this study was to investigate whether these four SNPs are associated with GC in Chinese Han population.
Genotype frequencies of these four SNPs of pre-miRNAs in 220 GC patients and 530 control subjects were performed using a PCR-RFLP assay.
No significant differences in genotype and allelic distribution were found in these four SNPs between GC and control subjects in the Chinese Han population. However, we found that the allelic frequency distributions of control subjects in these four SNPs were significantly different from those of the Japanese and the Koreans (All the P<0.05).
The four SNPs did not show any significant correlation with the development of GC in the Chinese Han population, based on the current study.
越来越多的证据表明，miRNAs 参与了胃癌（GC）发病机制中的各种遗传和表观遗传修饰。最近的研究集中在包括 rs11614913、rs3746444、rs2910164 和 rs2292832 在内的四个前 miRNA 单核苷酸多态性（SNP）上。这些四个 SNP 与 GC 的风险显著相关，并被描述为候选易感因素。然而，先前的结果显示存在相互矛盾的发现。本研究的目的是探讨这四个 SNP 是否与中国汉族人群的 GC 相关。
采用 PCR-RFLP 法检测 220 例 GC 患者和 530 例对照者这四个前 miRNA SNP 的基因型频率。
在汉族人群中，GC 组和对照组在这四个 SNP 的基因型和等位基因分布上无显著差异。然而，我们发现这四个 SNP 中对照组的等位基因频率分布与日本人及韩国人存在显著差异（均 P<0.05）。
基于本研究，这四个 SNP 与中国汉族人群 GC 的发生发展无明显相关性。
