Grisham William, Jones Heidi B, Park Sun Hee
Department of Psychology, University of California, Los Angeles, Los Angeles, CA 90095-1563.
J Undergrad Neurosci Educ. 2003 Fall;2(1):A28-35. Epub 2003 Oct 15.
This article describes a laboratory module taught at UCLA and offers digitized microscope images that will allow instructors to recreate this module at their home institutions with only a computer required. This module allows for 1) an exploration of the effects of hormones on neural development, 2) the demonstration of sex differences in the nervous system, 3) the production of robust and statistically significant data by novice undergraduates, 4) the discussion of sophisticated statistical analyses (ANOVAs with significant main effects and an interaction), and 5) the understanding of at least some of the neuroanatomy of the spinal cord. Specifically, this module both replicates and extends a previously published experiment on sexually dimorphic neurons in the spinal cord of rats (Grisham et al., 1992), which examined the effect of antiandrogen exposure (Flutamide) in utero on sexually dimorphic spinal motoneurons in male and female rats.
本文介绍了加州大学洛杉矶分校所教授的一个实验室模块，并提供了数字化显微镜图像，这将使教师仅需一台电脑就能在自己的院校重现该模块。这个模块能够实现以下几点：1）探究激素对神经发育的影响；2）展示神经系统中的性别差异；3）让本科新手学生生成可靠且具有统计学意义的数据；4）讨论复杂的统计分析（具有显著主效应和交互作用的方差分析）；5）理解脊髓的至少部分神经解剖结构。具体而言，该模块重复并扩展了之前发表的一项关于大鼠脊髓中性别二态性神经元的实验（格里森姆等人，1992年），该实验研究了子宫内抗雄激素暴露（氟他胺）对雄性和雌性大鼠性别二态性脊髓运动神经元的影响。
