Acker Corey D, Loew Leslie M
Center for Cell Analysis and Modeling, University of Connecticut Health Center, Farmington, CT, USA.
Methods Mol Biol. 2013;995:147-60. doi: 10.1007/978-1-62703-345-9_11.
In this protocol, we describe the procedures we have developed to optimize the performance of voltage-sensitive dyes for recording changes in neuronal electrical activity. We emphasize our experience in finding the best dye conditions for recording backpropagating action potentials from individual dendritic spines in a neuron within a brain slice. We fully describe procedures for loading the dye through a patch pipette and for finding excitation and emission wavelengths for the best sensitivity of the fluorescence signal to membrane voltage. Many of these approaches can be adapted to in vivo preparations and to experiments on mapping brain activity via optical recording.
在本方案中，我们描述了为优化电压敏感染料记录神经元电活动变化的性能而开发的程序。我们强调了在寻找最佳染料条件以记录脑片内单个神经元树突棘的反向传播动作电位方面的经验。我们全面描述了通过膜片吸管加载染料以及寻找激发和发射波长以实现荧光信号对膜电压最佳灵敏度的程序。这些方法中的许多都可适用于体内实验以及通过光学记录绘制脑活动的实验。
