Drug Metabolism & Toxicology Research Laboratories, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Nigeria.
Cell Biochem Funct. 2014 Jan;32(1):31-8. doi: 10.1002/cbf.2968. Epub 2013 Mar 12.
The present study investigated the protective effects of kolaviron, a biflavonoid from the seed of Garcinia kola, and vitamin E on ethylene glycol monoethyl ether (EGEE)-induced haematotoxicity and renal apoptosis in male rats. EGEE was administered at a dose of 200 mg kg(-1) alone or simultaneously administered with kolaviron (100 and 200 mgkg(-1) ) and vitamin E (50 mg kg(-1) ) for 14 days. Results of haematological examination showed that white blood cells, platelets, neutrophils and mean corpuscular haemoglobin concentration were significantly lower, whereas lymphocytes were increased in EGEE-exposed rats compared with those in the control. Administration of EGEE caused a significant decrease in the superoxide dismutase and catalase activities as well as in the glutathione level but significantly increased glutathione Stransferase activity and levels of hydrogen peroxide and lipid peroxidation in kidneys of rats compared with those in the control. Also, EGEE-treated rats showed significant elevation in the serum urea and creatinine with marked increase in the frequency of terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling assay-positive apoptotic cells in the tubular epithelial cells in comparison with the control. Co-administration with kolaviron or vitamin E exhibited chemoprotective effects against EGEE-mediated haematotoxicity, augmented renal antioxidant status and prevented the induction of renal apoptosis.
本研究调查了来自可乐果种子的双黄酮 kolaviron 和维生素 E 对乙二醇单乙醚 (EGEE) 诱导的雄性大鼠血液毒性和肾细胞凋亡的保护作用。EGEE 以 200mgkg(-1) 的剂量单独给药，或同时与 kolaviron（100 和 200mgkg(-1)）和维生素 E（50mgkg(-1)）一起给药 14 天。血液学检查结果表明，与对照组相比，EGEE 暴露组的白细胞、血小板、中性粒细胞和平均红细胞血红蛋白浓度显著降低，而淋巴细胞增加。与对照组相比，EGEE 处理的大鼠肾脏中超氧化物歧化酶和过氧化氢酶活性以及谷胱甘肽水平显著降低，谷胱甘肽 S-转移酶活性以及过氧化氢和脂质过氧化水平显著升高。此外，与对照组相比，EGEE 处理的大鼠血清尿素和肌酐水平显著升高，肾小管上皮细胞末端脱氧核苷酸转移酶介导的 dUTP 缺口末端标记法阳性凋亡细胞的频率也明显增加。与 kolaviron 或维生素 E 共同给药对 EGEE 介导的血液毒性具有化学保护作用，增强了肾脏抗氧化状态，并防止了肾细胞凋亡的诱导。
